CHARACTERIZATION AND IDENTIFICATION OF PSEUDO-NITZSCHIA SPECIES IN LAMPUNG BAY, INDONESIA

Authors

  • Arief Rachman
  • Hikmah Thoha

DOI:

https://doi.org/10.14203/mri.v46i1.602

Keywords:

diatom, Pseudo-nitzschia, taxonomy, TEM, key character

Abstract

The domoic acid producing Pseudo-nitzschia has received more attention in the recent years due to increasing amnesic shellfish poisoning (ASP) cases in many Asian countries. However, information on morphological characters of Pseudo-nitzschia species in Indonesia was very limited, which hinders the attempt to quickly identify the species during a harmful algal blooms in the coastal waters. Thus this study aimed to identify and characterize the Pseudo-nitzschia species found in Lampung Bay, Indonesia. Phytoplankton samples used in this study were taken from the reference collection for plankton (RCP) in Research Center for Oceanography, Indonesian Institute of Sciences (RCO-LIPI). The samples were collected from Padang Cermin area in Lampung, during high tide in 2005. In this research, morphology and morphometry of Pseudo-nitzschia were observed using light microscope (LM) and transmission electron microscope (TEM). The Pseudo-nitzschia species found in Lampung was labelled as LMP3. Jaccard cluster analysis, using simple average link, showed that LMP3 was Pseudo-nitzschia pungens. The morphology and morphometry of LMP3 were matched perfectly with P. pungens (100% similarity) and differed from other Pseudo-nitzschia species in seriata complex. Unfortunately, it was not known whether the P. pungens LMP3 in this study was the toxin producer strain as this study used preserved sample, thus it was not possible to detect any trace of domoic acid in there.     

Downloads

Download data is not yet available.

References

Almandoz, G., Hernando, M., & Ferrario, M. (2008). SEM observations of Pseudo-nitzschia from the Beagle Channel (Argentina): P. seriata in the southern hemisphere? Harmful Algae News, 38.

Barokah, G. R., Putri, A. K., & Gunawan, G. (2016). Kelimpahan Fitoplankton Penyebab Hab (Harmful Algal Bloom) di Perairan Teluk Lampung pada Musim Barat dan Timur. Jurnal Pascapanen dan Bioteknologi Kelautan dan Perikanan, 11(2), 115-126.

Casteleyn, G., Chepurnov, V. A., Leliaert, F., Mann, D. G., Bates, S. S., Lundholm, N., Rhodes, L., Sabbe, K. & Vyverman, W. (2008). Pseudo-nitzschia pungens (Bacillariophyceae): a cosmopolitan diatom species? Harmful Algae, 7(2), 241-257.

Casteleyn, G., Leliaert, F., Backeljau, T., Debeer, A-E, Kotaki, Y., Rhodes, L., Lundholm, N., Sabbe, K. & Vyverman, W. (2010). Limits to gene flow in a cosmopolitan marine planktonic diatom. Proceedings of the National Academy of Sciences, 1-6.

Cerino, F., Orsini, L., Sarno, D., Dell’Aversano, C., Tartaglione, L., & Zingone, A. (2005). The alternation of different morphotypes in the seasonal cycle of the toxic diatom Pseudo-nitzschia galaxiae. Harmful Algae, 4(1), 33-48.

Cupp, E. E. (1943). Marine plankton diatoms of the west coast of North America. Bulletin of The Scripps Institution of Oceanography, 5(1), 1-238.

Hallegraeff, G. (1994). Species of the diatom genus Pseudo-nitzschia in Australian waters. Botanica Marina, 37(5), 397-412.

Hasle, G. R. (1976) 6.3. Some specific preparations: 6.3.1. Diatoms. In Sournia, A. (Ed.). Phytoplankton Manual. United Nations Educational, Scientific, and Cultural Organization. Paris. France. pp. 137-142.

Hasle, G. R. (1993). Nomenclatural notes on marine planktonic diatoms. The family Bacillariaceae. Beih. Nova Hedwigia, 106, 315-321.

Hasle, G., Lange, C., & Syvertsen, E. (1996). A review of Pseudo-nitzschia, with special reference to the Skagerrak, North Atlantic, and adjacent waters. Helgoländer Meeresuntersuchungen, 50(2), 131.

Hernández-Becerril, D. U. (1998). Species of the planktonic diatom genus Pseudo-nitzschia of the Pacific coasts of Mexico. Hydrobiologia, 379(1-3), 77-84.

Lelong, A., Hégaret, H., Soudant, P. & Bates, S.S. 2012, Pseudo-nitzschia (Bacillariophyceae) species, domoic acid and amnesic shellfish poisoning: revisiting previous paradigms, Phycologia (51): 168-216.

McAleece, N., Gage, J. D., Lambshead, J., & Peterson, G. L. J. (1997). Biodiversity Professional Statistical Analysis Software. London: The Natural History Museum & Scottish Association of Marine Science.

Mizushima, K., Matsuoka, K., & Fukuyo, Y. (2007). Vertical distribution of Pyrodinium bahamense var. compressum (Dinophyceae) cysts in Ambon Bay and Hurun Bay, Indonesia. Plankton and Benthos Research, 2(4), 163-174.

Orsini, L., Sarno, D., Procaccini, G., Poletti, R., Dahlmann, J., & Montresor, M. (2002). Toxic Pseudo-nitzschia multistriata (Bacillariophyceae) from the Gulf of Naples: morphology, toxin analysis and phylogenetic relationships with other Pseudo-nitzschia species. European Journal of Phycology, 37(2), 247-257.

Pednekar, S. M., Bates, S. S., Kerkar, V., & Matondkar, S. P. (2018). Environmental Factors Affecting the Distribution of Pseudo-nitzschia in Two Monsoonal Estuaries of Western India and Effects of Salinity on Growth and Domoic Acid Production by P. pungens. Estuaries and Coasts, 41(5), 1448-1462.

Rachman, A. (2011). Laporan kegiatan UNESCO/IOC/WESTPAC training course on taxonomy and ecology of the diatom Pseudo-nitzschia (20-23 Maret 2011) di University Malaysia Sarawak, Malaysia. Unpublished report.

Rachman, A. (2013). Pseudo-nitzschia: Fitoplankton kosmopolit dan potensial toksik. Oseana, 38(1), 15-25.

Rivera, P. (1985). Las especies del género Nitzschia Hassall, sección Pseudo-nitzschia (Bacillariophyceae), en las aguas marinas chilenas. Gayana Bot, 42(3-4), 9-38.

Skov, J., Lundholm, N., Moestrup, O., & Larsen, J. (1999). Leaflet no. 185: Potentially toxic phytoplankton, the diatom genus Pseudo-nitzschia (Diatomophyceae / Bacillariophyceae). In J. A. Lindsey (Ed.), ICES identification leaflets for plankton (pp. 1-23). Denmark: International Council for The Exploration of the Sea.

Sidabutar, T. (2010). Kondisi fitoplankton di Teluk Jakarta pada periode 2008-2009. In R. Muchsin (Ed.), Dinamika ekosistem perairan Kepulauan Seribu, Teluk Jakarta. (pp. 121-138). Jakarta: Pusat Penelitian Oseanografi, Lembaga Ilmu Pengetahuan Indonesia.

Solihin, A., Hasani, Q., & Yulianto, H. (2015). Hubungan Perubahan Kualitas Air Dan Pertumbuhan Fitoplankton Berbahaya Pada Lingkungan Budidaya Ikan Di Perairan Ringgung Teluk Lampung. AQUASAINS, 3(2), 289-296.

Thessen, A. E., Dortch, Q., Parsons, M. L., & Morrison, W. (2005). Effect of salinity on Pseudo-nitzschia species (Bacillariophyceae) growth and distribution. Journal of Phycology, 41(1), 21-29.

Thoha, H. (2010). Studi variasi musiman terhadap kelimpahan fitoplankton di perairan Teluk Jakarta 2009. In R. Muchsin (Ed.), Dinamika ekosistem perairan Kepulauan Seribu, Teluk Jakarta. (pp. 139–146). Jakarta: Pusat Penelitian Oseanografi, Lembaga Ilmu Pengetahuan Indonesia.

Thoha, H., Rachman, A., Sidabutar, T., Fitriya, N., Bayu D., M., Muawanah, Iwataki, M., Takahashi, K. & Masseret, E. (2015). First Record of the Harmful dinoflagellate Cochlodinium polykrikoides in Lampung Bay, Indonesia. Harmful Algae News, 50, 14-15.

UNESCO/IOC/WESTPAC. (2011). Taxonomy and ecology of the diatom Pseudo-nitzschia. UNESCO IOC/WESTPAC Training workshop on taxonomy and ecology of the diatom Pseudo-nitzschia. Universiti Malaysia Sarawak.

Downloads

Published

2022-06-15

How to Cite

Rachman, A., & Thoha, H. (2022). CHARACTERIZATION AND IDENTIFICATION OF PSEUDO-NITZSCHIA SPECIES IN LAMPUNG BAY, INDONESIA. Marine Research in Indonesia, 46(1). https://doi.org/10.14203/mri.v46i1.602

Issue

Section

Original Research Articles