Effects of F/2 media modification on growth and antioxidant activity of microalga Nannochloropsis oculata

Authors

  • Mohammad Aulia Ghafari Marine Science Department, Faculty of Fisheries and Marine Science, Brawijaya University
  • Indyaswan Tegar Suryaningtyas Research Center for Oceanography, Indonesian Institute of Sciences (LIPI)
  • Defri Yona Marine Science Department, Faculty of Fisheries and Marine Science, Brawijaya University
  • Serly Sapulete Research Center for Oceanography, Indonesian Institute of Sciences (LIPI)
  • Nurhalis Tarmin Research and Development Division for Marine Bio Industry, Indonesian Institute of Sciences (LIPI)
  • La Ali Research Center for Oceanography, Indonesian Institute of Sciences (LIPI)
  • Ramli Marzuki Research and Development Division for Marine Bio Industry, Indonesian Institute of Sciences (LIPI)
  • Ratih Pangestuti Research and Development Division for Marine Bio Industry, Indonesian Institute of Sciences (LIPI)

DOI:

https://doi.org/10.14203/mri.v45i1.560

Keywords:

Microalgae, Antioxidant, F/2 Media, Nannochloropsis oculata

Abstract

Nannochloropsis oculata is a common microalga that can be found in marine water. One of the most commonly used media for microalga culture is F/2 media; however, the optimum F/2 media composition for N. oculata growth is rarely informed. Therefore, the aims of this study are to determine the effect of F/2 medium modification on the growth of  N. oculata and the antioxidant activity of N. oculata. This study consists of several procedures including; cultivation, growth observation (cell density and biomass), extraction, and antioxidant activity of N. oculata extracts. The highest growth (cell density and biomass) of N. oculata was found in media F/2 + 0.5 ml, while the lowest was in media F/2 - 0.5 ml. The strongest antioxidant activity of N. oculata was found in media F/2 + 1 ml, and the lowest was in the media F/2 - 0.5 ml. Media modification gave different results on cell density, biomass, and antioxidant activity of microalgae N. oculata.

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Author Biographies

Indyaswan Tegar Suryaningtyas, Research Center for Oceanography, Indonesian Institute of Sciences (LIPI)

Researcher at Microalgae Laboratory of Research Center for Oceanography, LIPI

Defri Yona, Marine Science Department, Faculty of Fisheries and Marine Science, Brawijaya University

Lecturer in Marine Science Department of Brawijaya University, Malang

Serly Sapulete, Research Center for Oceanography, Indonesian Institute of Sciences (LIPI)

Technician of Microalgae Laboratory, Research Center for Oceanography, LIPI

Ratih Pangestuti, Research and Development Division for Marine Bio Industry, Indonesian Institute of Sciences (LIPI)

Researcher at Marine Bioprospecting Laboratory, Research Center for Oceanography, LIPI

References

Abdurrachman, O., Mutiara, M., Buchori, L., 2013. Pengikatan Karbon Dioksida Dengan Mikroalga (Chlorella vulgaris, Chlamydomonas sp., Spirullina sp.) Dalam Upaya Untuk Meningkatkan Kemurnian Biogas. J. Teknol. Kim. Dan Ind. 2, 5.

Anam, C., Agustini, T.W., Romadhon, 2014. Pengaruh Pelarut Yang Berbeda Pada Ekstraksi Spirulina platensis Serbuk Sebagai Antioksidan Dengan Metode Soxhletasi. J. Pengolah. Dan Bioteknol. Has. Perikan. 3, 7.

Bariyyah, S.K., Hanapi, A., Fasya, A.G., Abidin, M., 2013. Uji Aktivitas Antioksidan Terhadap Dpph Dan Identifikasi Golongan Senyawa Aktif Ekstrak Kasar Mikroalga Chlorella sp. Hasil Kultivasi Dalam Medium Ekstrak Tauge. Alchemy. https://doi.org/10.18860/al.v0i0.2890.

Brahmantara, I.B.G., Anggreni, A.A.M.D., Gunam, I.B.W., 2015. Pengaruh Konsentrasi Penambahan Sodium Nitrat Dan Sodium Fosfat Pada Media Guillard Terhadap Konsentrasi Biomassa Mikroalga Nannochloropsis sp. J. Rekayasa Dan Manaj. Ind. 3, 9.

Dinata, K.D.W., Anggreni, A.A.M.D., Antara, N.S., 2017. Pengaruh Konsentrasi Natrium Nitrat dan Natrium Dehidrogen Fosfat pada Media Walne Terhadap Konsentrasi Biomassa dan Protein Nannochloropsis oculata. J. Rekayasa Dan Manaj. Agroindustri 5, 9.

Ebrahimzadeh, M.A., Khalili, M., Dehpour, A.A., 2018. Antioxidant activity of ethyl acetate and methanolic extracts of two marine algae, Nannochloropsis oculata and Gracilaria gracilis - an in vitro assay. Braz. J. Pharm. Sci. 54. https://doi.org/10.1590/s2175-97902018000117280.

Feller, R., Matos, Â.P., Mazzutti, S., Moecke, E.H.S., Tres, M.V., Derner, R.B., Oliveira, J.V., Junior, A.F., 2018. Polyunsaturated ?-3 and ?-6 fatty acids, total carotenoids and antioxidant activity of three marine microalgae extracts obtained by supercritical CO2 and subcritical n-butane. J. Supercrit. Fluids 133, 437–443. https://doi.org/10.1016/j.supflu.2017.11.015.

Hafsa, M.B., Ben, I., Garrab, M., Aly, R., Gagnon, J., Naghmouchi, K., 2017. Antimicrobial, antioxidant, cytotoxic and anticholinesterase activities of water-soluble polysaccharides extracted from microalgae Isochrysis galbana and Nannochloropsis oculata. J. Serbian Chem. Soc. 82, 509–522. https://doi.org/10.2298/JSC161016036B.

Hu, H., Gao, K., 2006. Response of Growth and Fatty Acid Compositions of Nannochloropsis sp. to Environmental Factors Under Elevated CO2 Concentration. Biotechnol. Lett. 28, 987–992. https://doi.org/10.1007/s10529-006-9026-6.

Mahat, K., Jamaluddin, H., Mohd. Zain, N.A., 2015. The Effect Of Different Phosphate Concentration On Growth, Lipid Productivity And Methyl Palmitate Methyl Ester Production By Nannochloropsis oculata. J. Teknol. 77. https://doi.org/10.11113/jt.v77.6915.

Muharni, Elfita, Amanda, 2013. Aktivitas Antioksidan Senyawa (+) Morelloflavon Dari Kulit Batang Tumbuhan Gamboge (Garcinia xanthochymus). Pros. Semirata FMIPA Univ. Lampung.

Reyimu, Z., Özçimen, D., 2017. Batch cultivation of marine microalgae Nannochloropsis oculata and Tetraselmis suecica in treated municipal wastewater toward bioethanol production. J. Clean. Prod. 150, 40–46. https://doi.org/10.1016/j.jclepro.2017.02.189.

Sangeetha, P., Anuradha, V., Suganya, V., Bhuvana, P., 2018. In Vitro Antioxidant And Radical Scavenging Activity Of Marine Microalga Nannochloropsis oculata. World J. Pharm. Pharm. Sci. 7, 15.

Sari, A.S.P., Ratnasari, E., Wisanti, 2012. Pengaruh Pemberian Jenis Pupuk yang Berbeda terhadap Laju Pertumbuhan Populasi dan Kadar Lemak Nannochloropsis oculata. Lentera Biol. 1, 7.

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Published

2020-07-27

How to Cite

Ghafari, M. A., Suryaningtyas, I. T., Yona, D., Sapulete, S., Tarmin, N., Ali, L., Marzuki, R., & Pangestuti, R. (2020). Effects of F/2 media modification on growth and antioxidant activity of microalga Nannochloropsis oculata. Marine Research in Indonesia, 45(1), 33–38. https://doi.org/10.14203/mri.v45i1.560

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